Prot Direct

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Direct Protein Dye-Staining

Normally Coomassie and similar dyes are used for staining proteins after SDS-, native electrophoresis or after isoelectric focusing.
Sensitivity should be 10 ng after SDS- or native electrophoresis and 100 ng after IEF.
Recipes are available with separate first fixing step. Others like stated here stain with one step.
Normally after staining a destaining of the background follows the staining step


			[Hot-Coomassie] [Semi-Colloidal Coom] [Colloidal Coomassie] [IEF-Coomassie] [Coomassie-Violet] [Blakesley/Roti-Blue] [Azurblue Stain] [Zinc Imidazol Stain] [Coom. Troubles SDS] [Coom. Troubles 2D]

	The dyes


	: SDS, native : SDS, native : SDS, native : IEF : IEF : native, IEF : denat.IEF : native, SDS

April 28, 2023

Molecule	Dye	Sensitivity	Info
SDS & Native proteins	Coomassie R 350 Sigma B4921-20TAB*	10 ng	...still the best!
IEF-proteins	Coomassie G 250 SERVA #17524.01 Coomassie-Violet 17 Sigma #210579-50g	100 ng 100 ng	hydrophilic proteins lipophilic proteins


	* 0,4 g dye per tablet


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)