This staining takes a little longer than the normal “Hot Coomassie Staining”. The advantages is: a) More sensitivity b) Also peptides are visible c) Also lipophylic proteins stay in the gel during staining d) Ideal for preparative 2D-stainings
The semi colloidal Coomassie-staining is staining and fixing at the same time. The Phosphoric acid is less lipophilic than the Acetic acid. This results in a slightly colloidal staining. The 3% Acetic Acid is only used for getting the Coomassie better solubilized and we achieve therefor a „tank-clean“ staining recipe. We recommend this method for peptides and preparative 2D-gels.
Stock solutions: (Volumes are given for normal sized gels, 12 × 26 cm) staining solution: 0.01 % (w/v) Coomassie R 350 (GE: 1 tablet) in 2.8 L H2Odist + 0.5% (w/v) Phosphoric acid (18 mL) + 3% HAc (90 ml HAc) —> fill up to 3 L destaining solutiont: 200 mL 10% HAc; 400 mL for large gels. preserving solution: 200 mL 10% (v/v) glycerol; 400 mL for large gels
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