Stock solutions Staining solution: 0.03% (w/v) Coomassie R-350 (Sigma B4921-20TAB*) 1 tablet (corresponds to 0.4 g dye substance) in 1,25 l 12.5% (v/v) acetic acid (HAc)
Sensitivity enhancement (in progress at EDC): Addition of 0.01% (w/v) Coomassie Violet R200 (Acid Violet; CI 42650, Sigma #210579-50g) + 0.00065% Ponceau S Example: 2 tablets R350 + 250 ml HAc + 2.250 l Bidest + 250 mg Coomassie Violet + 16 mg Ponceau S ---> staining 45 min; destaining #1: overnight, destaining #2 & 3: 2 x 60 min, preserving: 30 min
Destaining solution: 12.5% (v/v) HAc Nicest destaining (with no loss of lipophylic compounds) is: 12.5% HAc overnight, then 2 x 1 h Preserving solution: 5%-10%* (v/v) Glycerol (*depends on %T of the gel and the air-humidity
|