Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

Datenschutzerklärung >>

Two major problems occur:

1.   Blue stripes along the iso-pH-lines occur after staining and destaining
           Cause: The Ampholytes thet are creating the pH-gradients are also
             “Amin-Carbon-Acides” and though strongly stainable with Coomassie. See below.
              Remedy: Usa longer washing steps after the TCA and/or a higher alcohol concentration
              to get rid of it. See this page: bluelines

2.   Lipophylic Proteins were not stained (at all)
             Cause: After the separation and the fixation lipophilic proteins can get native again
             even after the gel swam 20 minutes in 20% TCA!
             Remedy: See this page: Lipoproteins

EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)