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All 2DGels can be stained very sensitive and quantitative.
(Attention: use the normal film supported gels, fluor films will not stay more than 30% ethanol!)
Stock solutions:
A) Coomassie Brillant Blue G250 Soluting (CBB-stock)
(5 g Coomassie Brillant Blue G-250, ad. 100mL with Bidest)
B) Colloidal Coomassie Staining Solution (CCD-stock)
(50 g (NH4)2SO4 + 6 ml 85% (w/v) Phosphoric acid, ad. 490mL with Bidest ad 10ml CBB-stock)
C) The final Colloidal Coomassie Solution (CCS-solution)
(200ml CCD-stock+ 50ml MeOH)
Prepare the Colloidal Coomassie Solution alwasy freshly!
Protocol:
2 h fixing in 40% ethanol, 10% Acedic acid
2 x 10 min washing in Bidest
1 day (15 h) staining in Colloidal Coomassie Solution (CCS)
2 x 15 min washing #1 in 20% methanol
2 x 1 h washing #2 with Bidest
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