Fast and reversible staining, very good compatible with mass spectrometry. Used also for storing of IPG-Strips when no cooling is available. We suggest to order: Roti-White from Carl Roth Karlsruhe Germany
Solutions: Solution a) 10 mL Roti-White I (10xconc) + 90 mL H2O dist. Solution b) 10 mL Roti-White II (10xconc) + 90 mL H2O dist. Solution c) 5 mL Roti-White I (10xconc) + 495 mL H2O dist. Solution d) 50 mM EDTA-Sodium salt + 25 mM TRIS (adjust pH to 8.3 with TRIS)
Staining: Rinse 1 x in H2O dist. Shake the strips for 15 min in a) Rinse 1 x in H2O dist. Shake the strips for ~3-4 min in b) (until the gel is white, the bands stay clear)
Storing: Rinse 1 x in H2O dist. Shake the strips in 150 mL c) for ~2 x 5 min (until you see white flocculation)
Melt the strips in plastic bags together with 2-3 mL c)
Mobilisation: Shake the strips in d) ~5 min (until the negative bands are nearly not anymore visible)
EDCElectrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)