wheat

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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April 28, 2023

Four methods are recommended:

1.   Special Cereal Typing Kit “Sortype” uses cathodal nativ electrophoresis pH 4.3:
For determination of wheat- and barley-varieties via their Prolamins (Gliadins), incl. malt, see below.
Also suitable for millet- rice- and maize-varieties
Malt-Method: A special extraction procedure is necessary for the malt-grains. Go to “Malt Method”
All sample treatments, see manual: Cereal typing with the Sortype Kit
2.   Protein Buffer Kit Cathodal and DryGel Elpho 12.5% 52S: uses native cathodal electrophoresis pH 5.5
For determination of wheat- and barley-varieties. Manual: Protein Buffer Kit Cathodic
3. IEFGel cereal 24S EQ-type, IEFGel cereal 40S EQ-type or IEFGel 104S cereal bidirect EQ-type
Produced with special SepaLytes Wheat (ProTec Heidelberg)
For determination of wheat- (and barley) -varieties
Manual: DryGel IEF cereal
4.   SDSGel 12.5% 40S EQ-type
Suitable also for determination of baking quality
Manual: ElphoGel Kit SDS


	IEF in cooperation with:



		SepaLytes

wheat varieties on IEFGel cereal 40S EQ-type (pH 4-6, half gel)


	[Wheat Native Elec.] [Standard Wheat 2002] [Sortype in practise] [Malt Method] [Demo Sample Prep] [Wheat IEF] [Wheat SDS]


	* * * * * IEF *


	* nativ cathodic electrophoresis IEF - isoelectric focusing * SDS-electrophoresis


	wheat varieties on native cathodal electrophoresis (Sortype gel pH 4.3)


	wheat varieties on native cathodal electrophoresis (Native Cathodal pH 5.5)


	wheat varieties on SDS-electrophoresis (SDSGel 12.5% 25S EQ-type), r = reduced samples - the other samples are not reduced


	wheat varieties on IEFGel cereal bidirect 104S EQ-type (pH 4-6). Run time 1.5 h


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)