Electro-blotting (western blotting): Normal gels have to be cut from the film support before starting the blotting process, otherwise no current will flow through the face of the gel. Soon available: EDC’s BlotGels. Here the support film can be rolled away easily without cutting first. BlotGel-IEF manual and Blotting of Horizontal Gels.
This method is recommended for all SDS-gels (10% - 12.5%T!)
Contact blotting This is the easiest way of blotting! Blotting by directed diffusion. But it will only gives you good results if the gel itself is thinner than 1 mm and below 10%T. This method is recommend for all IEF-gels. The efficiency of this method is not as high as with western blot methods.
See special application-note: “Blotting of Horizontal Gels”
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