Transfer buffers (Discontinuous buffer system) Anode solution I: 0.3 mol/L Tris (36.3 g), 20% (v/v) methanol200 mL, make up to 1 L with distilled water. Anode solution II: 25 mmol/L Tris (3.03 g), 20 %(v/v) methanol 200 mL, make up to 1 L with distilled water. Cathode solution: 40 mmol/L ε-aminocaproic acid (5.2 g), 0.01% (w/v) SDS0.1 g, 20% (v/v) methanol200 mL, make up to 1L with distilled water.
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