EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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contact-blotting

September 21, 2020

This is the easiest way of blotting! It will only gives you good results if the gel itself is thinner than 1 mm and not more than 12.5%T.
This method is recommend for all IEF-gels and ElphoGels .

To be flexiblewith sample volumes we recommend to use gels without slot
 (SDS: edc-4209). Application paper: NovaBlot ref 80-1106-19 (GE Healthcare)

SDSGel without slots

Picture and info courtesy: Karine L’Allemand ALK France


blotfolieeinlegKlein

1. A blotting foil is layed on the gel’s surface

glasauflegKlein

3. a glassplate follows

papierauflegKlein

2. 10-12 papers were set on top of the blot-foil

beschwerungKlein

4. 3-5 kg pressure for 2 h

Beo_Dry_Blotter

Alternative:Dyeagnostics BEO-DryBlotter