This is the easiest way of blotting! It will only gives you good results if the gel itself is thinner than 1 mm and not more than 12.5%T. This method is recommend for all IEF-gels and ElphoGels .
To be flexiblewith sample volumes we recommend to use gels without slot (SDS: edc-4209). Application paper: NovaBlot ref 80-1106-19 (GE Healthcare)
Picture and info courtesy: Karine L’Allemand ALK France
1. A blotting foil is layed on the gel’s surface
3. a glassplate follows
2. 10-12 papers were set on top of the blot-foil
4. 3-5 kg pressure for 2 h
Alternative:Dyeagnostics BEO-DryBlotter
EDCElectrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)