hotcoom2d

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Hot Coomassie Staining 2D

April 28, 2023


	The first two washing steps have to be performed when Pharmalytes or Biolytes were present in the first dimension. Servalytes or Sinulyte in the first dimension: gel goes directly in the hot staining solution!

solution (see hot coomassie)	0.4-0.7 mm	Volume normal gel*	0.8-1.0 mm	temp
40% EtOH / 10% HAc	2 h - overnight	200 ml	3 h - overnight	ambient
destaining solution	20 min	200 ml	30 min	ambient
fresh staining solution at 50-60 ^oC (see picture, fume cupboard!)	30 min	600 ml	60 min	50-60 ^oC
destaining solution in a tray (rocking platform)	overnight* + 2 x 30 min	200 ml	overnight* + 2 x 60 min	ambient
preserving solution (in a tray)	20 min	200 ml	30 min	ambient


	Air-drying: Overnight, gel should stand upright. Abbrev.: EtOH = Ethanol, HAc = Acetic Acid * overnight in the first destaining solution is optimal! * large size gels: double volumes



			DryGel Kit 2D normal size, hot coomassie-stained (+Coomassie Violet)

		EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)