hotcoom1D

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Hot Coomassie Staining 1D

April 28, 2023


	The gels go directly to the hot staining solution, no TCA necessary! 2D-gels: special page

solution (see hot coomassie)	0.4-0.7 mm	Volume normal gel*	0.8-1.0 mm	temp
fresh staining solution at 50-60 ^oC (see picture, fume cupboard!)	30 min	600 ml	60 min	50-60 ^oC
destaining solution in a tray (rocking platform)	overnight* + 2 x 30 min	200 ml	overnight* + 2 x 60 min	ambient
preserving solution (in a tray)	20 min	200 ml	30 min	ambient


	Air-drying: Overnight, gel should stand upright. Abbrev.: EtOH = Ethanol, HAc = Acetic Acid, TCA = Trichloroacetic Acide * overnight in the first destaining solution is optimal! * large gels: double volumes



		DryGel SDS 24S, hot coomassie-stained, half gel

				EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)