EDC’s new BlotGels can be used to perform a electro-blot because BlotGels can easily be removed from their support film. See EDC’s new manual.
The semidry blot method is recommended.
After the IEF the support film of the BlotGels should be removed first.
To do this first a dry nitrocellulose membrane 0.45 um (GVS North America, NitroPure, supported, #1212590) is fixed to the lab bench and the gel is placed on it, gel-facing down. The support film is now rolled away and the stack is taken to the blot apparatus. BlotGel IEF-manual.
Then the Immuno-Staining with an Anti-human IgG Antibody (HRP-conjugated, DAKO P0449) is performed on the membrane.
Advantage: A well introduced technique, IGG-selective staining.
Disadvantage: A additional technique (the blotting) has to be perfromed