electrophoresis-gels

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Februar 16, 2023

ElphoGels are Ready-To-Use gels for SDS and DNA with a thickness of 0,5 mm. Methods: SDS
These gels are polymerized on support films: Polyester for Coomassie and Silver stain, also for fluorescence in the red channel or
NF-film support for fluorescence on all channels.

EQ-type: A new matrix was developed! For best resolution and silver staining behavior. Comparable to the discontinued ExCelGels and to all self-casted SDS-gels!
Gel Electrodes: are now supplied with all ElphoGels SDS, non toxic! Best resolution and super sensitive silver-staining.
All film-supported gels can be scanned directly without any glass cassette! Increased sensitivity!
Stacking gel (5%T) and resolving gel (12.5%T).
PET support film:
For Coomassie- and silverstain. The fluorophores f.e. T-REX, Flamingo and Pro-Q are working in the red channel (exciting: 648 nm, emission: 663 nm)
See the manuals and data-sheets on “Downloads”. Staining recipes: “fluorescence staining”, “phosphorylated proteins”
NF support film:
For fluorescence stainings in all channels (UV, blue, green and red), e.g. DIGE, FITC etc.

All ElphoGels can be run on flatbed professional, also on EDC’s flatbed modular system




	Horizontal gel - on a plastic support film - also as EQ-type



				Gel Electrodes are supported with the ElphoGels SDS


	[ElphoGel SDS 25S] [ElphoGel SDS 52S] [ElphoGel SDS no slots] [ElphoGel SDS 7.5% 25S] [ElphoGel gradient] [ElphoGel SDS 25S NF] [ElphoGel SDS 52S NF] [ElphoGel gradient NF]


	Gel Electrodes for best resolution and silver staining


	flatbed professional runs also bidirectional




	EQ-type matrix for protein-SDS-mycelles



		Urinary Proteins: 104 samples run synchronously....bidirect run


	Ready-To-Use ElphoGel SDS Kits (gels on backing-films complete with Gel-Electrodes (SDS), Sample Buffer and Cooling Fluid)

name	order	gels, dimensions	slots	support	price (EUR)
ElphoGel SDS Kit 12.5% 25S EQ-type *	edc-4202	4 gels 250 x 110 x 0,5 mm	25 slots (à 15 ul)*	PET	215
ElphoGel SDS Kit 12.5% 52S EQ-type *	edc-4203	4 gels 250 x 110 x 0,5 mm	52 slots (à 5 ul)*	PET	215
ElphoGel SDS Kit 12.5% 52S	edc-4213	4 gels 250 x 85 x 0,5 mm	52 slots (à 5 ul)*	PET	215
ElphoGel SDS Kit 12.5% no slots EQ-type *	edc-4209	4 gels 250 x 110 x 0,5 mm	no slots	PET	215
ElphoGel SDS Kit 7.5% 25S EQ-type *	edc-4210	4 gels 250 x 110 x 0,5 mm	25 slots (à 15 ul)*	PET	215
Urinary Protein Kit Gradient **	edc-4010	4 gels 250 x 85 x 0,5 mm	52 slots (à 5 ul)*	PET	314


	10X Sample Buffer edc-4201 50 ml 25 EUR


		Also available: SDS 1D Gel Electrode Kit edc-5013 72 EUR

	10X Sample Buffer ...for diluted samples


	Ready-To-Use ElphoGel SDS Kits NF (gels on non fluorescent film with Gel-Electrodes (110 mm) or Electrode-strips (85 mm), Sample Buffer and Cooling Fluid)

name	order	gels, dimensions	slots	support	price (EUR)
ElphoGel SDS Kit 12.5% 25S NF EQ-type *	edc-4302	4 gels 250 x 110 x 0,5 mm	25 slots (à 15 ul)*	NF-film	239
ElphoGel SDS Kit 12.5% 52S NF EQ-type *	edc-4303	4 gels 250 x 110 x 0,5 mm	52 slots (à 5 ul)*	NF-film	239
ElphoGel SDS Kit Gradient 52S NF *	edc-4306	4 gels 250 x 85 x 0,5 mm	52 slots (à 5 ul)*	NF-film	334
ElphoGel DNA Kit Gradient 52S NF *	edc-xxxx	4 gels 250 x 85 x 0,5 mm	52 slots (à 5 ul)*	NF-film	334


	7,5% - 10% - 15% on NF-support: please ask *EDC*

	10X Sample Buffer SDS NF edc-4200 50 ml 25 EUR

		Additional necessary: DryPool Combi


		Also available: SDS/DNA 1D Gel-Electrode Kit edc-5013 79 EUR

	10X Sample Buffer NF ...for diluted samples (no dyes and an optimized pH-value for fluorescence derivating process)


	* All slot geometries are multipipette compatible * As kit with fluorescent prelabeling dyes: Dyeagnostics GmbH http://www.dyeagnostics.com/site/products/spl/, UFO-Kit


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)