SDS Elpho

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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SDS-Electrophoresis


	A denaturing electrophoresis for proteins with the following features: 1. All proteins (acidic and basic) run towards the anode 2. The proteins run according to their molecular mass 3. Also lypophylic (non water-soluble) proteins run


	[SDS-General] [SDS-Standards] [Hot-Coomassie] [SDS-Trouble]

April 28, 2023


			The SDS molecule

	Calculating the Rf-values of the SDS-proteins: stacking/separation gel

		Your target molecules -- which method?

Range Molecule	Method	Pattern
60 kD - 600 kD	SDS, 5%T	spreaded
20 kD - 300 kD	SDS, 7.5%T	spreaded
12 kD - 200 kD	SDS, 10%T	spreaded
10 kD - 150 kD	SDS, 12.5%T	spreaded
5 kD - 125 kD	SDS, 15%T	spreaded
5 kD - 600 kD	SDS, 7.5 - 20%T	compressed
Glycoproteins	SDS-Borate, 10%T or 15%	spreaded


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)