Staining of blotting-films should be done in special procedures and dyes
Oktober 18, 2021
Staining of the blot membranes
Fast Green staining Dissolve 0.1% (w/v) of Fast Green in 1% acetic acid; Stain for 5 min; destain the background with distilled water for 5 min; complete destaining of the bands is achieved by incubating the film for 5 min in 0.2 mol/L NaOH
PVDF-membrane - Fast Green stain
Ponceau S Staining Dissolve 0.1%(w/v) Ponceau S in 1 %(v/v) acetic acid; Stain for 5 min; Destain two times for 5 minutes with 5 % acetic acid.
Nitrocellulose - Ponceau stain
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