1dpharmalyte

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Hot Coomassie R350 or Sensitive Colloidal Coomassie G250 Stainings

Pharmalytes in the first dimension:
If Pharmalytes were used in the first dimension this 2 washing steps should be done first:

Washing programm: 40% EtOH/10% HAc for 30 min, 20% EtOH/10% HAc for 30 min

...then the two staining procedures above may follow:


	[Hot-Coomassie 2D]

April 28, 2023


	DryGel-2D normal size, 250 ug E.coli, Hot Coomassie staining with the additional washing steps before


	Colloidal Coomassie Staining Attention: use the normal film supported gels, non-fluorescent films will not stay more than 40% ethanol! Washing programm first: 40% EtOH/10% HAc for 30 min, 20% EtOH/10% HAc for 30 min


	[Colloidal Coomassie]


	2D-gel vertical (24 x 24 cm), 300 ug E.coli, Colloidal Coomassie Staining


	Semi Colloidal Coomassie Staining Washing programm first: 40% EtOH/10% HAc for 30 min, 20% EtOH/10% HAc for 30 min


	[Semi-Colloidal Coom]


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