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Dr. Hanspeter Schickle
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No Alkylation done after Reducing the Samples

Oktober 18, 2021

Most SDS-samples were reduced using Dithiothreitol or Mercaptoethanol.
When the samples have cooled down after heating 3 min 96°C, normally an alkylation has to be performed.
This alkylation prohibits re-oxydizing of the Disulfid-bridges.
Reoxydizing can end up in big-sized giga-molweight proteins sticking at the anodal slot-wall.
Mostly happening when the sample-volume is very small in its Eppi-cup.

Use the alkylation reaction after the reducing of the SDS-samples:


This reaction is described in EDC’s manuals:
ElphoGels SDS Kit, SDS DryGel Kit

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