Most SDS-samples were reduced using Dithiothreitol or Mercaptoethanol. When the samples have cooled down after heating 3 min 96°C, normally an alkylation has to be performed. This alkylation prohibits re-oxydizing of the Disulfid-bridges. Reoxydizing can end up in big-sized giga-molweight proteins sticking at the anodal slot-wall. Mostly happening when the sample-volume is very small in its Eppi-cup.
Remedy: Use the alkylation reaction after the reducing of the SDS-samples:
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