nfsupport

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Fluorescence Visualization in Horizontal Gels
on Non Fluorescent Gel-Support

April 28, 2023

The normally used polyester sheets show a high autofluorescence activity in the UV-channel and therefor they are not suitable for any UV-fluorescence visualization.
Non-fluorescence films have a low auto-fluorescence and so they can undergo fluorescence visualization procedure also in the UV-channel.
New dyes for the DIGE-techniques are developed: Refraction-2D™ from Dyeagnostics.
See the data-sheets and manuals on “Downloads”.
Coperation with Dyeagnostics (Halle, Germany)


	Application Areas:


	[Protein Fluorescence] [DIGE] [DNA Fluorescence]


	Scanning of film-backed gels Film-backed gels are scanned directly on the scanner’s platen with gel-side down. Non-fluorescent frame-bars are used to prevent curling of plastic backed gels during scanning. Suitable of both all large and normal format gels. 3 x 2 PVC-bars: 2 x 10x25x265mm; 2 x 10x25x190mm; 2 x 10x25x100mm




	scan-frames 3 x 2 frame-bars




		the scan-frames are holding down the edges of the film-backed gel during the scan-process


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)