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Due to a new dye (T-REX 310) - conjugated to the anti-human IgG antibody - the immunofluorescence staining methods getting possible with the same gel used also for silver-staining.
After 2 hours reaction with this Goat anti-human IgG (H+L) / T-Rex 310 and 3 hours washing out of the exceed conjugate, the gel goes directly to a Fluorescence-Imager.
The ReDye physical properties: 648 nm excitation, 663 nm emission.
Advantage: Quick, easy, sensitive and IgG-selective staining.
Disadvantage: Additional hardware is necessary.
Tested instruments:
Fujifilm: FLA 7000 and FLA 5100:
Settings: red laser, 40 um resolution, amplification 825 pmt, filter 630 nm
See the IEFGel 6-11 manual on “Downloads”, page 8.
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