Blue (Coomassie-staining) or black (silver-staining) curtain in the alkaline region
How to avoid the Pharmalytes-SDS complexes to be stained after the second dimension? Pharmalytes are optimal as addition in the first dimension. Here two remedies.....
April 03, 2025
Remedy #1: Stay with the Pharmalytes and use the additional washing steps given in the different staining methods: Hot Coomassie, Semi Colloidal Coomassie
Pharmalytes in the first dimension - Hot Coomassie Staining without the additional washing steps
Pharmalytes in the first dimension - Hot Coomassie Staining + the additional washing steps
Remedy #2: Use Servalytes / Sinulytes in a lower concentration in the first dimension:
Pharmalyte-SDS complexes in the second dimension (left side), silver-staining started with TCA (Trichloroacetic Acid)
Servalyte / Sinulyte / SepaLyte will not form SDS complexes in the second dimension
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