urininterpret

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Interpretation of Results: Urinary Proteins

April 28, 2023


	Please note that the urinary samples are not treated with a reducing agent, so the molecular weights are not exactly fitting to the marker lane (but the Immunoglobulins are running as complete molecules, ~ 160 kD).

Sample	Diagnosis	Protein-Conc.	Dilution
mol-weight standard Lonza #50547	225 kD—5 kD	275 ng per band in 15 µl	100 µl+ 100 µl
1	pure tubulary proteins	143 mg/l	no
2	glomerulary proteins - mid range selectivity	428 mg/l	no
3	glomerulary proteins - no selectivity	2140 mg/l	1+1**
4	mixed urinary proteins mostly tubulary	1200 mg/l	no
5	Bence-Jones proteins - free chains	180 mg/l	no
6	mixed urinary proteins	467 mg/l	no
7	native urin	115 mg/l	no
8	mixed urinary proteins mostly glomerulary	2500 mg/l	1+1**
9	mixed urinary proteins mostly tubulary + light chains	1082 mg/l	no
10	glomerulary proteins - with selectivity	132 mg/l	no
11	native urin - plus xxx	100 mg/l	no


	* samples are treated 450 µl Urine + 50 µl Sample Buffer 10X, 3 min 95°C ** samples are diluted 225 µl Urine + 225 µl H₂O dist. + 50 µl Sample Buffer 10x, 3 min 95°C


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