ElectrophoresisDevelopment & ConsultingDr. Hanspeter SchickleWolfgang Gstrein
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Interpretation of Results: Urinary Proteins
April 03, 2025
Please note that the urinary samples are not treated with a reducing agent, so the molecular weights are not exactly fitting to the marker lane (but the Immunoglobulins are running as complete molecules, ~ 160 kD).
Sample
Diagnosis
Protein-Conc.
Dilution
mol-weight standardLonza #50547
225 kD—5 kD
275 ng per band in 15 µl
100 µl+ 100 µl
1
pure tubulary proteins
143 mg/l
no
2
glomerulary proteins -mid range selectivity
428 mg/l
3
glomerulary proteins -no selectivity
2140 mg/l
1+1**
4
mixed urinary proteinsmostly tubulary
1200 mg/l
5
Bence-Jones proteins -free chains
180 mg/l
6
mixed urinary proteins
467 mg/l
7
native urin
115 mg/l
8
mixed urinary proteinsmostly glomerulary
2500 mg/l
9
mixed urinary proteinsmostly tubulary + light chains
1082 mg/l
10
glomerulary proteins -with selectivity
132 mg/l
11
native urin -plus xxx
100 mg/l
* samples are treated 450 µl Urine + 50 µl Sample Buffer 10X, 3 min 95°C** samples are diluted 225 µl Urine + 225 µl H2O dist. + 50 µl Sample Buffer 10x, 3 min 95°C
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