Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Symptom: A sample lane is spreaded lateral displacing their neighbour lanes.
Cause: When samples hold too much salt they are
a) accelerated lateral towards the gel without salt-loading, lateral sample spreading
b) SDS: unsharp band
Remedy:
1.   Tolerate this.
2.   IEF, native electrophoresis: Desalt sample using small columns like NAP-5 (GE 17-0853-01)

3. SDS: Precipitate the proteins with 20% TCA. Centrifuge samples. Wash 1 x with dist.water. Centrifuge. Resolubilize with sample buffer.
Additional advantage beside the desalting effect: sample concentrations can be increased easily!

EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)