doubledimension

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Double Dimension Electrophoresis

April 28, 2023


	Alternative suggestion to classical 2D: Double-Dimensional-2D (if the basic IPG-focusing results are not good) First dimension = cathodal native Electrophoresis using an acidic buffer. Second dimension = SDS-electrophoresis.


	Second dimension: SDS


	First dimension: Native cathodal electrophoresis


	+


	Cathodal acidic buffer. (Peter Reiter, Gelita, Eberbach, Germany)



			Collagen-hydrolysates: the smaller peptides migrate quicker than the bigger even in native electrophoresis


			5-7 kD

		11 kD

				3 kD

	-


	Hot Coomassie gives nice bands: Smallest marker protein is 10.000


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)