ElectrophoresisDevelopment & ConsultingDr. Hanspeter SchickleWolfgang Gstrein
Datenschutzerklärung >>
Double Dimension Electrophoresis
April 28, 2023
Alternative suggestion to classical 2D: Double-Dimensional-2D (if the basic IPG-focusing results are not good)First dimension = cathodal native Electrophoresis using an acidic buffer.Second dimension = SDS-electrophoresis.
Second dimension: SDS
First dimension: Native cathodal electrophoresis
+
Cathodal acidic buffer.(Peter Reiter, Gelita, Eberbach, Germany)
Collagen-hydrolysates: the smaller peptides migrate quicker than the biggereven in native electrophoresis
5-7 kD
11 kD
3 kD
-
Hot Coomassie gives nice bands: Smallest marker protein is 10.000
EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)