In all DNA-electrophopresis methods the DNA run negatively charged to the anode! There are 3 different main DNA-electrophoresis methods:
Native samples run in native gels: d(ouble) s(dranded)-DNA electrophorese ds: Easy method, 1 fragment = 1 band, but no exact molecular weights (because of the double-stranded conformation polymorphisme)
Denaturated samples run in denaturated gels: s(ingle)-s(tranded) denatured electrophoresis ss: Difficult method, 1 fragment = 2 bands (PCR-anomaly), but exact molecular weights (method used in all sequencers)
Denaturated samples run in native gels: s(ingle)-s(tranded) conformation polymorphisme electrophoresis sscp: No molecular weights but sequence-related run (= mutation-detection)
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