acidicpeptides

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Acidic Peptides


	Acidic peptides could be easily separated in IEF, native and in SDS-electrophoresis.


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			E.coli in native electrophoresis



		Native electrophoresis displays better at the anodal end. Migration speed is not relative to the mol-weight!


	E.coli in SDS-electrophoresis




	Peptides not only at the anodal end!


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		Glyco-peptides (milk) in anodal native electrophoresis



			Gel 15%T, native buffer pH 7.4. Ingolf Krause (Weihenstephan, Germany)

April 28, 2023


	Two dimensional electrophoresis beside the classical 2D: double native 2D First dimension = IPG-IEF pH 4-7, 11 cm Second dimension = anodal native electrophoresis native buffer


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	pH 4


	-----IPG-strip 4-7-----


	pH 7


	Double Native electrophoresis of 1 mg E.coli. First dimension: IPG-strip pH 4-7, 11 cm Second dimension: 12.5%R, native anodal buffer pH 7.4 Visualization: Hot Coomassie


	migration speed is not relative to the molweight


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)