ElectrophoresisDevelopment & ConsultingDr. Hanspeter SchickleWolfgang Gstrein
Acidic peptides could be easily separated in IEF, native and in SDS-electrophoresis.
E.coli in native electrophoresis
Native electrophoresis displays better at the anodal end.Migration speed is not relative to the mol-weight!
E.coli in SDS-electrophoresis
Peptides not only at the anodal end!
Glyco-peptides (milk) in anodal native electrophoresis
Gel 15%T, native buffer pH 7.4.Ingolf Krause (Weihenstephan, Germany)
September 21, 2020
Two dimensional electrophoresis beside the classical 2D: double native 2DFirst dimension = IPG-IEF pH 4-7, 11 cmSecond dimension = anodal native electrophoresis native buffer
Double Native electrophoresis of 1 mg E.coli.First dimension: IPG-strip pH 4-7, 11 cmSecond dimension: 12.5%R, native anodal buffer pH 7.4Visualization: Hot Coomassie
migration speed is not relative to the molweight