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Dr. Hanspeter Schickle
Wolfgang Gstrein

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Trouble Shooting Oligo IgG

Cathode is burning:
If more than ~ 12 native CSF-samples (undiluted, NaCL = 0.9% (w/v) ==> 150 mM !) were pipetted on the gel then we have too much conductivity in the electric field.
Remedy: Reduce the Voltage in the last step of the running conditions from 1900 to 1500 Volts

too much salt

Cathode has burned at 2 different places and the lanes started to run uneven

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