1. Popping up of the gels during automated staining process:
Silver staining is mostly done in the Autostainer from GE.
From time to time (12.5%T gels more than 10%T gels) gels will popp up during staining: certain aereas of the gel will then not be in contract of the different solution and will not stain properly.
Use the magnetic bars supplied with the automat.
These bars will hold the gels at the bottom of the staining tray.