Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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1.  Popping up of the gels during automated staining process:

Silver staining is mostly done in the Autostainer from GE.
From time to time (12.5%T gels more than 10%T gels) gels will popp up during staining: certain aereas of the gel will then not be in contract of the different solution and will not stain properly.

Remedy:

Use the magnetic bars supplied with the automat.
These bars will hold the gels at the bottom of the staining tray.

2.  Gel-film is producing a yellow / brown background:

Remedy: The use of Benzenesulfonic Acid (DNA-silver staining recipes) is recommended also for protein-separations.
The recipe: EDC-SDS Silver Staining. Please see also DNA-Silver Trouble

Optimization of the new conditioner and the silvering solution using increasing amounts of Benzenesulfonic Acid

EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)