Do not extract your proteins directly from the raw materials using detergent (SDS) -containing buffers! Correct extraction procedure:
Extraction buffer: 0.25 M TRIS, 0.2M Glycine, 0.001 M EDTA (pH 9.1), detergent max. 0.05%
Extraction procedure: 15 min ultra sonic treatment - 10 min centrifugation 10 000 rpm - take the supernatant!
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