Like every other separation system, electrophoresis has its optimal working concentration. If the sample concentration is too high, then the gel-buffer cannot hold the pH-value anymore. This gel is shortly before burning!
Protein concentrations: Not more than 1 ug/ ul when Coomassie stained! Dilute 1:50 when silver staining follows the SDS-electrophoresis
As a sufficient pre-test the protein test-stick “Albym-Test” (Boehringer) can be used; available in every pharmacy (for urinary proteins).
Protein Test Stick
Protein Concentration
Stick Colour
Sample-Dilution
negative
no dilution
0.3 g/l
no dilution
1.0 g/l
10ul + 30ul
5.0 g/l
10ul + 90ul
EDCElectrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)