step

reagent 

volume

time
(0.4-0.6 mm)

time
(1 mm)

1 Fixing

 40% (v/v) Ethanol / 10% (v/v) Acetic Acid
(160 ml EtOH / 40 ml HAc in 400 ml)
(place gel onto the solution in a glas tray, gel swims surface side down)

2 x 200 ml

2 x 20 min

2 x 30 min

2 Incubation

30% (v/v) Ethanol; 0.03% (w/v) Sodium Thiosulphate
10 mM Sodium Acetate; 0.125 % Glutardialdehyd (w/v)
(60 ml EtOH / 65 mg Thiosulfate / 300 mg NaAc / 1 ml Glutardialdehyde in 200ml)

200 ml

30 min

60 min

3 - 5 Washing

H₂O dist (place gel on the glas tray bottom surface side up)

3 x 200 ml

3 x 20 min

3 x 30 min

6 Silvering

0.25 % (w/v) AgNO₃ /0.03% (v/v) Formaldehyde
(500 mg AgNO₃ (or 10 ml stock sol.) / 180 µl Formaldehyde in 200 ml)

200 ml

40 min

50 min

7,8 Washing

H₂O dist

2 x 200 ml

2 x 1 min

2 x 10 min

10 Developing

2.5 % Na₂CO₃ (w/v) _{/ 0.03% (v/v) Formaldehyde / 0.00075% (w/v) Sodium-Thiosulfate}
(5 g NaCO₃ / 180 ul Formaldehyde / 75 ul Sodium-Thiosulfate (2%, fresh!) in 200 ml)

2 x 200 ml

3 - 5 min

 10 - 20 min

11 Stopping
Preserving

10% (v/v) HAc, 10% (v/v) Glycerol
(20 ml HAc / 20 ml Glycerol in 200 ml)

200 ml

20 min

40 min

Drying

air dry , on the support-film
then roll on a polyester-sheet

---

16 h

3 h
then cover
with a plastic film