IEF Markers

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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		IEF-Standards

	Dilutions* of the standards are given when as a visualization process Coomassie-staining is chosen. For silver-staining dilute further 1:25.

Product	Dilution* (marker + buffer)	Prestained ?	Loading	Order Number
GE 3-10 pH	200 ul per bottle	---	1 ug in 25 ul	17-0471-01
Biorad 4.65 - 9.6	10 ul + 240 ul	YES	1 ug in 25 ul	161-0310
Native Protein Mix (see below)	150 ul + 150 ul	Myoglobin, Cytochrom C	~ 1.3 ug in 25 ul	- self mix -

April 28, 2023


	Native Protein-Mix: each 1 mg / 10 ml. Can be used in both directions, anodal and cathodal. Should look like GE’s IEF Mix 3-10. Amyloglucosidase - pI 3.5, Glucose oxidase - pI 4.2, Trypsin inhibitor - pI 4.5, β-Lactoglobulin - pI 5.15 / 5.3 Carboanhydrase - pI 6.0, Myoglobin horse - pI 6.9 / 7.35, Lentil lectin - pI 7.75 / 8.0 / 8.3, Ribonuclease A - pI 9.45 Cytochrome C - pI 10.65


	IEFGel 3-10, Coomassie-Violet stained Standard: GE 3-10


	IEFGel 3-10, during run BioRad 4.6-9.6


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