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Double Native Electrophoresis

When the first dimension is a IEF (isoelectric focusing) and the second dimension is a native electrophoresis (anodal or cathodal - instead of the SDS-dimension of a 2D), this combination is called “Double Native Electrophoresis”.

First dimension is an IEF

double native

Second dimension is an anodal native

September 21, 2020

The second dimension does not display the molweights of the proteins, but 2 proteins with the same isoelectric point and different secondary or tertiary structure will be separated.

double nativ - molweights