DNA-Marker

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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		DNA-Standards

	This is a list of different DNA-standards

April 28, 2023

Product	Dilution Silver native (marker + buffer***)	Load**** (per band)	Dilution Silver *denat* (marker + 50% Formamide**)	Load**** (per band)	Dilution Fluorescence (native)	Load**** (per band)	Dilution Direct Stain (marker + buffer***)	Load**** (per band)	Dilution Direct Stain denat (marker + 50% Formamide**)	Load**** (per band)	Order #
Promega 100 bp	30 ul + 720 ul	2.5 ng in 6 ul	10 ul + 140 ul	5 ng in 6 ul	not good!		10 + 10	60 ng in 6 ul			G210A
BioRad 100 bp	10 + 990	12 ng in 6 µl			10 + 490	24 in 6µl	10 + 390	30 ng in 6 µl			170-8206
Invitrogen 50 bp	10 ul + 990 ul	3 ng in 6 ul	10 ul + 300 ul	10 ng in 6 ul	10 + 190	15 ng in 6 ul	10 + 90	35 ng in 6 ul	10 + 40	75 ng in 6 ul	10416-014
Cambrex 20 bp	40 ul + 500 ul	4 ng in 6 ul	not good!		not good!		10 + 15	40 ng in 6 ul			50320
Invitrogen 10 bp	10 ul + 700 ul	--	10 ul + 200 ul	--	not good!		10 + 40	--	10 + 20	--	10821-015
Amersham 100 bp	10 ul + 1290 ul		not good!		10 + 240		not tested				27-407-01
Amersham 1kb	10 ul + 990 ul		not good!		10 + 190		not tested				27-404-01
Promega Hinf 1	10 ul + 1840 ul		10 ul + 740 ul		not good!		not tested				G1751
Roth Quant 200	10 ul + 590 ul	10 ng in 6 ul	not yet tested!		10 + 120		10 ul + 90 ul	60 ng in 6 ul	20 ul + 80 ul	120 ng in 6 ul	QS200

****Load: According to the manufactor's figures in the manuals.
***Native: The dilution buffer should be the gel-buffer + 0.005% Xylene Cyanol.
**Denaturing: The dilution solution should be 50% Formamide + 0.005% Xylene Cyanol.
*10% (v/v) Dye: The fluorescence dye-solution should be diluted with DMSO.
Results: The markers should be visible easily in the above given dilution. Please see the native separation and the denaturing result below.


	Silver staining: Gel 15%T run native with a special buffer pH 7.4. About 3 ng band


	Silver staining: Gel 15%T run denaturing* with a special buffer pH 8.3, containing 6 M Urea. About 3 ng per band


	Fluorescence: Gel 10%T, run native with a special buffer pH 8.3, staining with GelStar. About 50 ng per band


	Direct DNA stain: Gel 10%T, run native with a special buffer pH 7.4, staing with Stains All. Concentrated lanes hold about 30 ng per band


	Direct DNA-Stain: Gel 15%T, run semidisc denatured with a special buffer pH 8.3, staining with Stains All. About 60 ng per band.


	EDC Electrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)