Support-films of other suppliers have an Agarose-layer between the film and the active coating. There parts the proteins run quicker than in the gel itself. showing the socalled forward-striking.
Special support-filmsdo not have this layer and should not show this effect!
Spots show forward-striking
forward-striking (center lane) in 1D-SDS
EDCElectrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)