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DIGE: Double Spots

In DIGE-technique the proteins were derivated with a fluorophor before the electrophoresis procedure. Of course this will also increase a little the mol-weight of these new molecules.
Down to 10 kD this will be no bigger problem in fluorescence scanning.
In the peptide region, from 10 kD downward real double spots could be visualized.

Be aware of this!

DIGE-derivated peptides visualized after the SDS-run with Lava Purple

April 28, 2023


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