Air is a very bad conductor of electric current. The IPG-strips should not have any bubbles underneath themselves.
When the cathodal buffer is floated into the chamber, already holding the cassettes, then some air-bubbles could be caught between the strip and the second dimension gel. Better is to pre-float the single cassettes with a pipette before the cathodal buffer is filled in
A long air bubbles is sitting between the IPG-strip and the second dimension gel
Pre-floating of the cassettes prevent catching air bubbles
April 28, 2023
EDCElectrophoresis Development & Consulting, Vor dem Kreuzberg 17, 72070 Tübingen (Germany)
