EDC Impressum & DSE

Electrophoresis
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Dr. Hanspeter Schickle
Wolfgang Gstrein

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Interpretation of Results: Urinary Proteins

Mai 30, 2018

Please note that the urinary samples are not treated with a reducing agent, so the molecular weights are not exactly fitting to the marker lane (but the Immunoglobulins are running as complete molecules, ~ 160 kD).

Urine_2Klein

Sample

Diagnosis

Protein-Conc.

Dilution

mol-weight standard
Lonza #50547

225 kD—5 kD

275 ng per band in 15 Ál

100 Ál+ 100 Ál

1

pure tubulary proteins

143 mg/l

no

2

glomerulary proteins -
mid range selectivity

428 mg/l

no

3

glomerulary proteins -
no selectivity

2140 mg/l

1+1**

4

mixed urinary proteins
mostly tubulary

1200 mg/l

no

5

Bence-Jones proteins -
free chains

180 mg/l

no

6

mixed urinary proteins

467 mg/l

no

7

native urin

115 mg/l

no

8

mixed urinary proteins
mostly glomerulary

2500 mg/l

1+1**

9

mixed urinary proteins
mostly tubulary + light chains

1082 mg/l

no

10

glomerulary proteins -
with selectivity

132 mg/l

no

11

native urin -
plus xxx

100 mg/l

no

* samples are treated 450 Ál Urine + 50 Ál Sample Buffer 10X, 3 min 95░C
** samples are diluted 225 Ál Urine + 225 Ál H2O dist. + 50 Ál Sample Buffer 10
x, 3 min 95░C