EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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single-stranded DNA-Electrophoresis SSCP

Mai 30, 2018

denaturated samples (50% Formamide, 95°C for 3 minutes, then chilled on ice) run on native gels, showing no molecular weight but:
s(ingle) s(tranded)-c(onformation) p(olymorphisme) = SSCP.
Sequence-related electrophoresis!

Samples 1-5 are denaturated. Although same mol-weights the 2 single strands per gene-locus show different separations (Wildtype - Mutant)

special gel with a special buffer system