EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Trouble Shooting Silver Stain

[Silver-Trouble]

Mai 30, 2018

Popping up of the gels during automated staining process:

Silver staining is mostly done in the Autostainer from GE.
From time to time (12.5%T gels more than 10%T gels) gels will popp up during staining: certain aereas of the gel will then not be in contract of the different solution and will not stain properly.

Remedy:

Use the magnetic bars supplied with the automat.
These bars will hold the gels at the bottom of the staining tray.

Gel has floating up during the silvering solution. No magnetic bars were used!

Silver-TS: floating up
Autostn

GE’s Autostainer