EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Silver Staining

Juli 13, 2018

Silver-staining is the most sensitive staining method known for proteins and DNA-fragments. Results of the silver staining cannot be used for quantification because the ODs of the bands do not correspond  linearely to the applied amount of the molecules.

[General]
[DNA-Silver]
[IEF Silver]
[IgG-silver-stain]
[SDS Silver]
[SDS Silver EDC]
[2D Silver]
[Droplet Test]
[Trouble Shooting AG]


DNA-fragments
IEF, neutral & acidic proteins
IEF, basic proteins
SDS gels, vertical
for EDC’s SDS- & 2D-gels
2D-gels, no Glutaraldehyd!
 

Molecule

Dye

Sensitivity

Info

nucleic acids

AgNO3

100 pg

No quantification possible

proteins

AgNO3

300 pg

No quantification possible

Autostainer

GE's Automated Silver Staining Automat