EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Run is Too Short

Mai 30, 2018

In EDC’s SDS buffer-system the SDS runs behind the Bromophenolblue (BPB). This means: Stopping the run with the BPB will left ~1.5 cm SDS in the separation gel.

Remedy:
Let the SDS run out of the gel and use the separation distance for your proteins by optimized shutdown:
   a) You can see the backside of the SDS-zone:
       mark it with Conchenille in the sample buffer.
       Let it out in the anodal strip, then shutdown.
   b) Use a prestained standard and run it till the 10 kDa stays just before the anodal strip.

See also 2D-trouble-shooting: “optimal shutdown

run longer!
Till the SDS is completely in the anode

Pfeil180
TroubleTooShortBearb

14 kDa

SDS

BPB

LonzaColor

Run stopped correctly

SinusFolie600_2KleinAS

Biozym standard:
“QuadColor”
(right lane)

run till the
green 10 kDa protein
is before the strip!