EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Striking Bands
Glycoproteins or Very Lipophylic Proteins

Mai 30, 2018

Some proteins, mostly Glycoproteins, show a special behavior in electrophoresis. In the case they are strong concentrated the bands are wavy shaped.
Glycoproteins are partly build out of sugar. SDS will not bind to this part of the molecule.
This causes striking bands because the starting process is too quick for them.
Remedy is to perform a slow start of the electrophoresis (mA and W minus 50% in phase #1).
Also addition of Boric acid (250 mM) to the sample buffer can help by charging the sugar of Glycoproteins nagatively.

TSGlyco1
Pfeil

Normal start voltage and mA

TSGlyco2

Start voltage and mA halved

sample buffer incl. 250 mM Boric Acid

Phase

Volts

mA

W

time

1

320

20

10

45 min

2

650

30

20

15 min

3

1200

42

40

2h 15 min