EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Basic Proteins

Mai 30, 2018

The molecule SDS is an anionic tenside and should dock to the proteins with its lipophylic tail.
Basic proteins are charged positively and though the SDS-molecule dock with its negative head - the sulfate group - to the proteins.
This will not enhance the negative charges of this mycelles which leads to a bad behavior in SDS-electrophoresis.

Remedy is to change to a TRIS-based buffer-system (ElphoGels) or, the best:

Run a native cathodic electrophoresis (native cathoda buffer kit, DryGel Elpho)
Please note that basic proteins will like this applied acidic buffer system....

Samples with SDS, followed by
silver-staining (Collagen-hydrolystes)

CollHydrolPH55SDSAgKlein

basic proteins don’t like SDS...

-

arrowgr2

+

Same samples with cathodal acidic buffer

CollHydrolPH55Klein

 3 kD

11 kD

5-7 kD

Collagen-hydrolysates: nice separation

+

-

Drawback is that in native electrophoresis proteins run more according to their charges than to their molweight!