EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

Datenschutzerklärung >>

SDS - Standards

Dilutions of the standards are given when as a visualization process Coomassie-staining is chosen.
Fluorescence: dilute 10 + 30. For silver-staining dilute 10 + 90.

For calculating the Rf-values: Standards can also be used to define the parting line between stacking and separation gel.

See special page: stack/separation gel

Product

Dilution Coomassie
(marker + gel-buffer)

Prestained ?

Loading
(per band)

15%
Long distance?

Order Number

Lonza: SDS 5-225 kD

100 ul + 300 ul

---

185 ng in 15 ul

yes

50547

Biozym: QuadColor 4.6 - 315 kD

100 ul + 300 ul

Yes

~185 ng in 15 ul

yes

830537

GE: HMW 66-669 kD

700 ul per bottle

---

~380 ng in 15 ul

yes

17-0445-01

BioRad: Dual Colour 10-250 kD

100 ul + 150 ul

YES

~600 ng in 15 ul

no

161-0374

Sigma: Ultra Low Range
1,060-26,600

10 ul + 490 ul

---

?

---

M3546

Sigma: 2,500-17,000
Peptide Molecular Weight Marker

3 ml per bottle

---

?

---

MWSDS17S

Sigma Mucin (big sized proteins)

 

 

 

 

M 3895

SDS-gel 12.5%T, Coomassie stained

SDS

SDS-gel 12.5%T, Dual Color standard during run

IEF

BioRad, Lonza standard

LonzaMarkerKlein

Lonza standard,
Sigma Low & Peptide

Lonza_26_17PlusKlein

Mai 30, 2018

Biozym standard,
ProSieve Quadcolor

LonzaColor