EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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SDS-Electrophoresis

A denaturing electrophoresis for proteins with the following features:
1.   All proteins (acidic and basic) run towards the anode
2.   The proteins run according to their molecular mass
3.   Also lypophylic (non water-soluble) proteins run

[SDS-General]
[SDS-Standards]
[Hot-Coomassie]
[SDS-Trouble]

Mai 30, 2018

sdsmol02

The SDS molecule

Calculating the Rf-values of the SDS-proteins: stacking/separation gel

Your target molecules -- which method?

Range Molecule

Method

Pattern

60 kD -  600 kD

SDS, 5%T

spreaded

20 kD - 300 kD

SDS, 7.5%T

spreaded

12 kD -  200 kD

SDS, 10%T

spreaded

10 kD -  150 kD

SDS, 12.5%T

spreaded

5 kD - 125 kD

SDS, 15%T

spreaded

5 kD - 600 kD

SDS, 7.5 - 20%T

compressed

Glycoproteins

SDS-Borate, 10%T
or 15%

spreaded