EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Direct Protein Dye-Staining

Normally Coomassie and similar dyes are used for staining proteins after SDS-, native electrophoresis or after isoelectric focusing.
Sensitivity should be 10 ng after SDS- or native electrophoresis and 100 ng after IEF.
Recipes are available with separate first fixing step. Others like stated here stain with one step.
Normally after staining a destaining of the background follows the staining step

[Hot-Coomassie]
[Semi-Colloidal Coom]
[Colloidal Coomassie]
[IEF-Coomassie]
[Coomassie-Violet]
[Blakesley/Roti-Blue]
[Azurblue Stain]
[Zinc Imidazol Stain]
[Coom. Troubles SDS]
[Coom. Troubles 2D]

The dyes

: SDS, native
: SDS, native

: SDS, native
: IEF
: IEF
: native, IEF
: denat.IEF
: native, SDS
 

Juli 02, 2018

Molecule

Dye

Sensitivity

Info

SDS & Native proteins

Coomassie R 350 Sigma B4921-20TAB*

10 ng

...still the best!

IEF-proteins

Coomassie G 250 SERVA #17524.01
Coomassie-Violet 17 Sigma #210579-50g

100 ng
100 ng

hydrophilic proteins
lipophilic proteins

* 0,4 g dye per tablet