EDC Impressum & DSE

Electrophoresis
Development & Consulting
Dr. Hanspeter Schickle
Wolfgang Gstrein

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Fluorescence Visualization in Horizontal Gels
 on Non Fluorescent Gel-Support

Mai 30, 2018

The normally used polyester sheets show a high autofluorescence activity in the UV-channel and therefor they are not suitable for any UV-fluorescence visualization.
Non-fluorescence films have a low auto-fluorescence and so they can undergo fluorescence visualization procedure also in the UV-channel.
New dyes for the DIGE-techniques are developed: Refraction-2D™ from Dyeagnostics.
See the data-sheets and manuals on “Downloads”.

Dyagnostics Logo
01-10-2010_NHD_Refraction-2D_grün_outlines
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Application Areas:

[Protein Fluorescence]
[DIGE]
[DNA Fluorescence]
ETC12_5%sKlein
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Scanning of film-backed gels
Film-backed gels are scanned directly on the scanner’s platen with gel-side down.
Non-fluorescent frame-bars are used to prevent curling of  plastic backed gels during scanning. Suitable of both all large and normal format gels.
3 x 2 PVC-bars: 2 x 10x25x265mm; 2 x 10x25x190mm; 2 x 10x25x100mm

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scan-frames
3 x 2 frame-bars

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the scan-frames are holding down the edges of the
film-backed gel during the scan-process