2D-gels are normally scanned within their cassettes. This leads to a loss of sensitivity: “reactive films”.
Special SDS-gels, 2D-DryGels, are polymerized on non-fluorescenting support-films and can easily be scanned directly lying on the scanner’s glass-plate:
Increasing of intensity will be achieved by factor 1.3 - 1.5!
Another method of increasing sensitivity is the use of new dye-technologie
Refraction-2D from Dyeagnostics. Data-sheets and manual see “Downloads”