After the IEF first a simple contact-blotting is done.
A Nitrocellulose membrane 0.45 um is pressed on the gel's surface (with some papers behind) for 45 min.
Then the Immuno-Staining with an Anti-human IgG Antibody (HRP-conjugated, DAKO P0449) is performed on the membrane.
Advantage: A well introduced technique, IGG-selective staining, no additional hardware.
Disadvantage: A additional technique (the blotting) has to be perfromed